Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000011557 (60%)
Mouse ENSMUSG00000056054 (56%)
Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Immunotopographical Differences of Human Skin
Front Immunol , 2018 Mar 5; 9:424. Epub 2018 Mar 5
Comparative Proteomic Analysis Reveals Activation of Mucosal Innate Immune Signaling Pathways during Cholera
Infect Immun , 2015 Mar; 83(3):1089-1103. Epub 2015 Feb 13
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