Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
In situ protein detection with enhanced specificity using DNA-conjugated antibodies and proximity ligation
Modern Pathology , September 22, 2017
Molecular transitions from papillomavirus infection to cervical precancer and cancer: Role of stromal estrogen receptor signaling
Proc Natl Acad Sci U S A , 2015 Jun 23; 112(25):E3255-E3264. Epub 2015 Jun 8
Snail Is a Direct Target of Hypoxia-inducible Factor 1α (HIF1α) in Hypoxia-induced Endothelial to Mesenchymal Transition of Human Coronary Endothelial Cells
J Biol Chem , 2015 May 13; 290(27):16653-16664. Epub 2015 May 13
Decidual PTEN expression is required for trophoblast invasion in the mouse
Am J Physiol Endocrinol Metab , 2010 Dec; 299(6):E936-E946. Epub 2010 Sep 21
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