Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000039552 (41%)
Rat ENSRNOG00000058561 (37%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
A quantitative super-resolution imaging toolbox for diagnosis of motile ciliopathies.
Sci Transl Med , 2020 Mar 18; 12(535)
Accuracy of Immunofluorescence in the Diagnosis of Primary Ciliary Dyskinesia
Am J Respir Crit Care Med , 2017 Jul 1; 196(1):94-101. Epub 2017 Jul 1
Immunofluorescence Analysis and Diagnosis of Primary Ciliary Dyskinesia with Radial Spoke Defects
Am J Respir Cell Mol Biol , 2015 Oct; 53(4):563-573
RSPH3 Mutations Cause Primary Ciliary Dyskinesia with Central-Complex Defects and a Near Absence of Radial Spokes
Am J Hum Genet , 2015/07/02; 97(1):153-162
Targeted NGS gene panel identifies mutations in RSPH1 causing primary ciliary dyskinesia and a common mechanism for ciliary central pair agenesis due to radial spoke defects
Hum Mol Genet , 2014 Jul 1; 23(13):3362-3374. Epub 2014 Feb 11
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