Enhanced Validation

Anti-RSPH4A Antibody

radial spoke head 4 homolog A (Chlamydomonas)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human RSPH4A
Alternative Gene Names
CILD11, dJ412I7.1, FLJ37974, RSHL3, RSPH6B
Price
$445.00
Product Number
HPA031196
Unit Size
Concentration
Lot dependent
Availability
On Demand
Ships
On Demand
Target Protein
radial spoke head 4 homolog A (Chlamydomonas)
Target Gene
RSPH4A
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
RPWEGKTAASPQYSEPESSEPLEAKQGPETGRQSRSSRPWSPQSRAKTPLGGPAGPETSSPAPVSPREPSSSP
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000039552 (41%)

Rat ENSRNOG00000058561 (37%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
radial spoke head 4 homolog A (Chlamydomonas)
Gene Name
RSPH4A
Alternative Gene Names
CILD11, dJ412I7.1, FLJ37974, RSHL3, RSPH6B
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000111834 on the Human Protein Atlas

Liu Z, Nguyen QPH, Guan Q, Albulescu A, Erdman L, Mahdaviyeh Y, Kang J, Ouyang H, Hegele RG, Moraes T, Goldenberg A, Dell SD, Mennella V

A quantitative super-resolution imaging toolbox for diagnosis of motile ciliopathies.

Sci Transl Med , 2020 Mar 18; 12(535)

PubMed ID: 32188719 DOI: 10.1126/scitranslmed.aay0071

Shoemark A, Frost E, Dixon M, Ollosson S, Kilpin K, Patel M, Scully J, Rogers AV, Mitchison HM, Bush A, Hogg C

Accuracy of Immunofluorescence in the Diagnosis of Primary Ciliary Dyskinesia

Am J Respir Crit Care Med , 2017 Jul 1; 196(1):94-101. Epub 2017 Jul 1

PubMed ID: 28199173 DOI: 10.1164/rccm.201607-1351OC

Frommer A, Hjeij R, Loges NT, Edelbusch C, Jahnke C, Raidt J, Werner C, Wallmeier J, Große-Onnebrink J, Olbrich H, Cindrić S, Jaspers M, Boon M, Memari Y, Durbin R, Kolb-Kokocinski A, Sauer S, Marthin JK, Nielsen KG, Amirav I, Elias N, Kerem E, Shoseyov D, Haeffner K, Omran H

Immunofluorescence Analysis and Diagnosis of Primary Ciliary Dyskinesia with Radial Spoke Defects

Am J Respir Cell Mol Biol , 2015 Oct; 53(4):563-573

PubMed ID: 25789548 DOI: 10.1165/rcmb.2014-0483OC

Jeanson L, Copin B, Papon JF, Dastot-Le Moal F, Duquesnoy P, Montantin G, Cadranel J, Corvol H, Coste A, Désir J, Souayah A, Kott E, Collot N, Tissier S, Louis B, Tamalet A, de Blic J, Clement A, Escudier E, Amselem S, Legendre M

RSPH3 Mutations Cause Primary Ciliary Dyskinesia with Central-Complex Defects and a Near Absence of Radial Spokes

Am J Hum Genet , 2015/07/02; 97(1):153-162

PubMed ID: 26073779 DOI: 10.1016/j.ajhg.2015.05.004

Onoufriadis A, Shoemark A, Schmidts M, Patel M, Jimenez G, Liu H, Thomas B, Dixon M, Hirst RA, Rutman A, Burgoyne T, Williams C, Scully J, Bolard F, Lafitte JJ, Beales PL, Hogg C, Yang P, Chung EM, Emes RD, O'Callaghan C, UK10K, Bouvagnet P, Mitchison HM

Targeted NGS gene panel identifies mutations in RSPH1 causing primary ciliary dyskinesia and a common mechanism for ciliary central pair agenesis due to radial spoke defects

Hum Mol Genet , 2014 Jul 1; 23(13):3362-3374. Epub 2014 Feb 11

PubMed ID: 24518672 DOI: 10.1093/hmg/ddu046

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