Enhanced Validation

Anti-RSPH1 Antibody

radial spoke head 1 homolog (Chlamydomonas)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human RSPH1
Alternative Gene Names
CILD24, FLJ32753, RSP44, RSPH10A, TSGA2
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
radial spoke head 1 homolog (Chlamydomonas)
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000057862 (90%)

Mouse ENSMUSG00000024033 (88%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-RSPH1 HPA016816.

Protein Name
radial spoke head 1 homolog (Chlamydomonas)
Gene Name
Alternative Gene Names
CILD24, FLJ32753, RSP44, RSPH10A, TSGA2
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000160188 on the Human Protein Atlas

Tu C, Nie H, Meng L, Yuan S, He W, Luo A, Li H, Li W, Du J, Lu G, Lin G, Tan YQ

Identification of DNAH6 mutations in infertile men with multiple morphological abnormalities of the sperm flagella

Sci Rep , 2019 Nov 1; 9:15864. Epub 2019 Nov 1

PubMed ID: 31676830 DOI: 10.1038/s41598-019-52436-7

Coutton C, Martinez G, Kherraf ZE, Amiri-Yekta A, Boguenet M, Saut A, He X, Zhang F, Cristou-Kent M, Escoffier J, Bidart M, Satre V, Conne B, Fourati Ben Mustapha S, Halouani L, Marrakchi O, Makni M, Latrous H, Kharouf M, Pernet-Gallay K, Bonhivers M, Hennebicq S, Rives N, Dulioust E, Touré A, Gourabi H, Cao Y, Zouari R, Hosseini SH, Nef S, Thierry-Mieg N, Arnoult C, Ray PF

Bi-allelic Mutations in ARMC2 Lead to Severe Astheno-Teratozoospermia Due to Sperm Flagellum Malformations in Humans and Mice

Am J Hum Genet , 2019 Jan 24; 104(2):331-340. Epub 2019 Jan 24

PubMed ID: 30686508 DOI: 10.1016/j.ajhg.2018.12.013

Shoemark A, Frost E, Dixon M, Ollosson S, Kilpin K, Patel M, Scully J, Rogers AV, Mitchison HM, Bush A, Hogg C

Accuracy of Immunofluorescence in the Diagnosis of Primary Ciliary Dyskinesia

Am J Respir Crit Care Med , 2017 Jul 1; 196(1):94-101. Epub 2017 Jul 1

PubMed ID: 28199173 DOI: 10.1164/rccm.201607-1351OC

Jeanson L, Copin B, Papon JF, Dastot-Le Moal F, Duquesnoy P, Montantin G, Cadranel J, Corvol H, Coste A, Désir J, Souayah A, Kott E, Collot N, Tissier S, Louis B, Tamalet A, de Blic J, Clement A, Escudier E, Amselem S, Legendre M

RSPH3 Mutations Cause Primary Ciliary Dyskinesia with Central-Complex Defects and a Near Absence of Radial Spokes

Am J Hum Genet , 2015/07/02; 97(1):153-162

PubMed ID: 26073779 DOI: 10.1016/j.ajhg.2015.05.004

Onoufriadis A, Shoemark A, Schmidts M, Patel M, Jimenez G, Liu H, Thomas B, Dixon M, Hirst RA, Rutman A, Burgoyne T, Williams C, Scully J, Bolard F, Lafitte JJ, Beales PL, Hogg C, Yang P, Chung EM, Emes RD, O'Callaghan C, UK10K, Bouvagnet P, Mitchison HM

Targeted NGS gene panel identifies mutations in RSPH1 causing primary ciliary dyskinesia and a common mechanism for ciliary central pair agenesis due to radial spoke defects

Hum Mol Genet , 2014 Jul 1; 23(13):3362-3374. Epub 2014 Feb 11

PubMed ID: 24518672 DOI: 10.1093/hmg/ddu046

Kott E, Legendre M, Copin B, Papon JF, Dastot-Le Moal F, Montantin G, Duquesnoy P, Piterboth W, Amram D, Bassinet L, Beucher J, Beydon N, Deneuville E, Houdouin V, Journel H, Just J, Nathan N, Tamalet A, Collot N, Jeanson L, Le Gouez M, Vallette B, Vojtek AM, Epaud R, Coste A, Clement A, Housset B, Louis B, Escudier E, Amselem S

Loss-of-Function Mutations in RSPH1 Cause Primary Ciliary Dyskinesia with Central-Complex and Radial-Spoke Defects

Am J Hum Genet , 2013 Sep 5; 93(3):561-570

PubMed ID: 23993197 DOI: 10.1016/j.ajhg.2013.07.013

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