Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000020752 (73%)
Rat ENSRNOG00000005107 (72%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Low expression of RecQ-like helicase 5 is associated with poor prognosis in patients with gastric cancer
Oncol Lett , 2019 Nov 21; 19(1):985-991. Epub 2019 Nov 21
Altered RECQL5 expression in urothelial bladder carcinoma increases cellular proliferation and makes RECQL5 helicase activity a novel target for chemotherapy
Oncotarget , 2016 Oct 15; 7(46):76140-76150. Epub 2016 Oct 15
Clinicopathological and prognostic significance of RECQL5 helicase expression in breast cancers
Carcinogenesis , 2015 Nov 19; 37(1):63-71. Epub 2015 Nov 19
Altered RECQ Helicase Expression in Sporadic Primary Colorectal Cancers
Transl Oncol , 2013 Aug; 6(4):458-469. Epub 2013 Aug 1
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
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