Anti-PTRH2 Antibody

peptidyl-tRNA hydrolase 2
Recommended Applications
Product Description
Polyclonal Antibody against Human PTRH2
Alternative Gene Names
BIT1, CFAP37, CGI-147, PTH2
Price
$445.00
Product Number
HPA012897
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
peptidyl-tRNA hydrolase 2
Target Gene
PTRH2
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
KSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGIGPGPADLIDKVTGHLKL
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000004288 (83%)

Mouse ENSMUSG00000072582 (81%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20 - 1:50

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
peptidyl-tRNA hydrolase 2
Gene Name
PTRH2
Alternative Gene Names
BIT1, CFAP37, CGI-147, PTH2
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000141378 on the Human Protein Atlas

Yao X, Jennings S, Ireland SK, Pham T, Temple B, Davis M, Chen R, Davenport I, Biliran H

The Anoikis Effector Bit1 Displays Tumor Suppressive Function in Lung Cancer Cells

PLoS One , 2014; 9(7):e101564. Epub 2014 Jul 8

PubMed ID: 25003198 DOI: 10.1371/journal.pone.0101564

Brunquell C, Biliran H, Jenings S, Ireland SK, Chen R, Ruoslahti E

TLE1 is an anoikis regulator and is downregulated by Bit1 in breast cancer cells

Mol Cancer Res , 2012 Nov; 10(11):1482-1495. Epub 2012 Sep 4

PubMed ID: 22952044 DOI: 10.1158/1541-7786.MCR-12-0144

Karmali PP, Brunquell C, Tram H, Ireland SK, Ruoslahti E, Biliran H

Metastasis of Tumor Cells Is Enhanced by Downregulation of Bit1

PLoS One , 2011; 6(8):e23840. Epub 2011 Aug 23

PubMed ID: 21886829 DOI: 10.1371/journal.pone.0023840

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Corresponding Antigens

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