Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000047250 (93%)
Rat ENSRNOG00000007415 (90%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Cytoplasmic PPARγ is a marker of poor prognosis in patients with Cox-1 negative primary breast cancers
J Transl Med , 2020 Feb 21; 18:94. Epub 2020 Feb 21
Understanding the Interplay between COX-2 and hTERT in Colorectal Cancer Using a Multi-Omics Analysis
Cancers (Basel) , 2019 Oct 11; 11(10):1536. Epub 2019 Oct 11
Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma.
Br J Dermatol , 2008 Mar; 158(3):527-38. Epub 2008 Jan 30
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