Enhanced Validation

Anti-PRKAR2B Antibody

protein kinase, cAMP-dependent, regulatory, type II, beta
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human PRKAR2B
Alternative Gene Names
Product Number
Unit Size
Lot dependent
On Demand
On Demand
Target Protein
protein kinase, cAMP-dependent, regulatory, type II, beta
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000009079 (94%)

Mouse ENSMUSG00000002997 (93%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20 - 1:50

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
protein kinase, cAMP-dependent, regulatory, type II, beta
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000005249 on the Human Protein Atlas

Godbole A, Lyga S, Lohse MJ, Calebiro D

Internalized TSH receptors en route to the TGN induce local Gs-protein signaling and gene transcription

Nat Commun , 2017 Sep 5; 8:443. Epub 2017 Sep 5

PubMed ID: 28874659 DOI: 10.1038/s41467-017-00357-2

Weigand I, Ronchi CL, Rizk-Rabin M, Dalmazi GD, Wild V, Bathon K, Rubin B, Calebiro D, Beuschlein F, Bertherat J, Fassnacht M, Sbiera S

Differential expression of the protein kinase A subunits in normal adrenal glands and adrenocortical adenomas

Sci Rep , 2017 Mar 13; 7:49. Epub 2017 Mar 13

PubMed ID: 28250426 DOI: 10.1038/s41598-017-00125-8

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Corresponding Antigens


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