Anti-PREX1 Antibody

phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
References (11)
Recommended Applications
Product Description
Polyclonal Antibody against Human PREX1
Alternative Gene Names
KIAA1415, P-REX1
Open Datasheet
Price
$450.00
Product Number
HPA001927
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
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[1 pictures]
Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
Target Gene
PREX1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
CAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEARASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEKIVEP
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000006952 (78%)

Mouse ENSMUSG00000039621 (78%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Protein Name
phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
Gene Name
PREX1
Alternative Gene Names
KIAA1415, P-REX1
UniProt ID
Q8TCU6
Gene (Ensembl)
ENSG00000124126
Entrez Gene ID
Entrez gene: 57580
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000124126 on the Human Protein Atlas

Cervantes-Villagrana RD, Color-Aparicio VM, Reyes-Cruz G, Vázquez-Prado J

Protumoral bone marrow-derived cells migrate via Gβγ-dependent signaling pathways and exhibit a complex repertoire of RhoGEFs

J Cell Commun Signal , 2019 Jan 5; 13(2):179-191. Epub 2019 Jan 5

PubMed ID: 30612298 DOI: 10.1007/s12079-018-00502-6

Adame-García SR, Cervantes-Villagrana RD, Orduña-Castillo LB, del Rio JC, Gutkind JS, Reyes-Cruz G, Taylor SS, Vázquez-Prado J

cAMP-dependent activation of the Rac guanine exchange factor P-REX1 by type I protein kinase A (PKA) regulatory subunits

J Biol Chem , 2018 Dec 10; 294(7):2232-2246. Epub 2018 Dec 10

PubMed ID: 30530493 DOI: 10.1074/jbc.RA118.006691

Cervantes-Villagrana RD, Adame-García SR, García-Jiménez I, Color-Aparicio VM, Beltrán-Navarro YM, König GM, Kostenis E, Reyes-Cruz G, Gutkind JS, Vázquez-Prado J

Gβγ signaling to the chemotactic effector P-REX1 and mammalian cell migration is directly regulated by Gαq and Gα13 proteins

J Biol Chem , 2018 Nov 16; 294(2):531-546. Epub 2018 Nov 16

PubMed ID: 30446620 DOI: 10.1074/jbc.RA118.006254

Rosmaninho P, Mükusch S, Piscopo V, Teixeira V, Raposo AA, Warta R, Bennewitz R, Tang Y, Herold‐Mende C, Stifani S, Momma S, Castro DS

Zeb1 potentiates genome‐wide gene transcription with Lef1 to promote glioblastoma cell invasion

EMBO J , 2018 Jun 14; 37(15):e97115. Epub 2018 Jun 14

PubMed ID: 29903919 DOI: 10.15252/embj.201797115

Marotti JD, Muller KE, Tafe LJ, Demidenko E, Miller TW

P-Rex1 Expression in Invasive Breast Cancer in relation to Receptor Status and Distant Metastatic Site

Int J Breast Cancer , 2017 Jun 15; 2017:4537532. Epub 2017 Jun 15

PubMed ID: 28698809 DOI: 10.1155/2017/4537532

Marei H, Carpy A, Woroniuk A, Vennin C, White G, Timpson P, Macek B, Malliri A

Differential Rac1 signalling by guanine nucleotide exchange factors implicates FLII in regulating Rac1-driven cell migration

Nat Commun , 2016 Feb 18; 7:10664. Epub 2016 Feb 18

PubMed ID: 26887924 DOI: 10.1038/ncomms10664

Chávez-Vargas L, Adame-García SR, Cervantes-Villagrana RD, Castillo-Kauil A, Bruystens JG, Fukuhara S, Taylor SS, Mochizuki N, Reyes-Cruz G, Vázquez-Prado J

Protein Kinase A (PKA) Type I Interacts with P-Rex1, a Rac Guanine Nucleotide Exchange Factor: EFFECT ON PKA LOCALIZATION AND P-Rex1 SIGNALING

J Biol Chem , 2016 Jan 21; 291(12):6182-6199. Epub 2016 Jan 21

PubMed ID: 26797121 DOI: 10.1074/jbc.M115.712216

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.

Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24

PubMed ID: 23435261 DOI: 10.1038/nmeth.2377

Campbell AD, Lawn S, McGarry LC, Welch HC, Ozanne BW, Norman JC

P-Rex1 Cooperates with PDGFRβ to Drive Cellular Migration in 3D Microenvironments

PLoS One , 2013; 8(1):e53982. Epub 2013 Jan 30

PubMed ID: 23382862 DOI: 10.1371/journal.pone.0053982

Lindsay CR, Lawn S, Campbell AD, Faller WJ, Rambow F, Mort RL, Timpson P, Li A, Cammareri P, Ridgway RA, Morton JP, Doyle B, Hegarty S, Rafferty M, Murphy IG, McDermott EW, Sheahan K, Pedone K, Finn AJ, Groben PA, Thomas NE, Hao H, Carson C, Norman JC, Machesky LM, Gallagher WM, Jackson IJ, Van Kempen L, Beermann F, Der C, Larue L, Welch HC, Ozanne BW, Sansom OJ

P-Rex1 is required for efficient melanoblast migration and melanoma metastasis

Nat Commun , 2011 Nov 22; 2:555. Epub 2011 Nov 22

PubMed ID: 22109529 DOI: 10.1038/ncomms1560

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Corresponding Antigens
PrEST Antigen PREX1 (APrEST86391)

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