Enhanced Validation

Anti-POLD2 Antibody

polymerase (DNA directed), delta 2, accessory subunit
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human POLD2
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
polymerase (DNA directed), delta 2, accessory subunit
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000014098 (96%)

Mouse ENSMUSG00000020471 (94%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
polymerase (DNA directed), delta 2, accessory subunit
Gene Name
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000106628 on the Human Protein Atlas

Cui Y, Keles S, Charbonnier LM, Julé AM, Henderson L, Celik SC, Reisli I, Shen C, Xie WJ, Schmitz-Abe K, Wu H, Chatila TA

Combined Immunodeficiency due to a loss of function mutation in DNA Polymerase Delta 1

J Allergy Clin Immunol , 2019 Oct 16; 145(1):391-401.e8. Epub 2019 Oct 16

PubMed ID: 31629014 DOI: 10.1016/j.jaci.2019.10.004

Conde CD, Petronczki ÖY, Baris S, Willmann KL, Girardi E, Salzer E, Weitzer S, Ardy RC, Krolo A, Ijspeert H, Kiykim A, Karakoc-Aydiner E, Förster-Waldl E, Kager L, Pickl WF, Superti-Furga G, Martínez J, Loizou JI, Ozen A, van der Burg M, Boztug K

Polymerase δ deficiency causes syndromic immunodeficiency with replicative stress

J Clin Invest , 2019 Aug 26; 129(10):4194-4206. Epub 2019 Aug 26

PubMed ID: 31449058 DOI: 10.1172/JCI128903

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Corresponding Antigens


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