Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000025608 (43%)
Rat ENSRNOG00000012495 (41%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Podocalyxin-like protein as a predictive biomarker for benefit of neoadjuvant chemotherapy in resectable gastric and esophageal adenocarcinoma
J Transl Med , 2018 Oct 24; 16:290. Epub 2018 Oct 24
Podocalyxin influences malignant potential by controlling epithelial–mesenchymal transition in lung adenocarcinoma
Cancer Sci , 2017 Apr 3; 108(3):528-535. Epub 2017 Apr 3
Expression of podocalyxin-like protein is an independent prognostic biomarker in resected esophageal and gastric adenocarcinoma
BMC Clin Pathol , 2016 Jul 29; 16:13. Epub 2016 Jul 29
Prognostic and predictive significance of podocalyxin-like protein expression in pancreatic and periampullary adenocarcinoma
BMC Clin Pathol , 2015; 15:10. Epub 2015 May 30
Podocalyxin Is a Marker of Poor Prognosis in Pancreatic Ductal Adenocarcinoma.
PLoS One , 2015; 10(6):e0129012
Proteome-wide Epitope Mapping of Antibodies Using Ultra-dense Peptide Arrays
Mol Cell Proteomics , 2014 Jun; 13(6):1585-1597. Epub 2014 Apr 4
Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer
Br J Cancer , 2013 Jun 11; 108(11):2321-2328. Epub 2013 May 7
Sialofucosylated podocalyxin is a functional E- and L-selectin ligand expressed by metastatic pancreatic cancer cells
Am J Physiol Cell Physiol , 2012 Sep 15; 303(6):C616-C624. Epub 2012 Jul 18
Methylation of an intronic region regulates miR-199a in testicular tumor malignancy.
Oncogene , 2011 Aug 4; 30(31):3404-15. Epub 2011 Mar 7
Overexpression of podocalyxin-like protein is an independent factor of poor prognosis in colorectal cancer.
Br J Cancer , 2011 Aug 23; 105(5):666-72. Epub 2011 Aug 9
Significant association and synergistic adverse prognostic effect of podocalyxin-like protein and epidermal growth factor receptor expression in colorectal cancer
J Transl Med , 1/01/01; 14:128. Epub 2016 May 10
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