Enhanced Validation

Anti-PKP1 Antibody

plakophilin 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human PKP1
Alternative Gene Names
B6P
Price
$450.00
Product Number
HPA027221
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
plakophilin 1
Target Gene
PKP1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
YTVRNLMASQPQLAKQYFSSSMLNNIINLCRSSASPKAAEAARLLLSDMWSSKELQGVLRQQGFDRNMLGTLAGANSLRNFTSRF
Verified Species Reactivity
Human, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000026413 (84%)

Rat ENSRNOG00000013728 (26%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.

Protein Name
plakophilin 1
Gene Name
PKP1
Alternative Gene Names
B6P
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000081277 on the Human Protein Atlas

Galindo I, Gómez-Morales M, Díaz-Cano I, Andrades Á, Caba-Molina M, Miranda-León MT, Medina PP, Martín-Padron J, Fárez-Vidal ME

The value of desmosomal plaque-related markers to distinguish squamous cell carcinoma and adenocarcinoma of the lung

Ups J Med Sci , 2019 Dec 6; 125(1):19-29. Epub 2019 Dec 6

PubMed ID: 31809668 DOI: 10.1080/03009734.2019.1692101

Kaz A, Luo Y, Dzieciatkowski S, Chak A, Willis J, Upton M, Leidner R, Grady W

Aberrantly methylated PKP1 in the progression of Barrett’s esophagus to esophageal adenocarcinoma

Genes Chromosomes Cancer , 2012 Apr; 51(4):384-393. Epub 2011 Dec 14

PubMed ID: 22170739 DOI: 10.1002/gcc.21923

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Join the Explorer Program

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Alternative Antibodies
Corresponding Antigens

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