Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Hexokinases link DJ-1 to the PINK1/parkin pathway
Mol Neurodegener , 2017 Sep 29; 12:70. Epub 2017 Sep 29
PHGDH as a key enzyme for serine biosynthesis in HIF2α-targeting therapy for renal cell carcinoma
Cancer Res , 2017 Sep 26; 77(22):6321-6329. Epub 2017 Sep 26
Modulating the therapeutic response of tumours to dietary serine and glycine starvation
Nature , 544, 372-376 (2017)
Validating and enabling phosphoglycerate dehydrogenase (PHGDH) as a target for fragment-based drug discovery in PHGDH-amplified breast cancer
Oncotarget , 2016 Aug 22; 9(17):13139-13153. Epub 2016 Aug 22
Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth
Sci Rep , 2016 Jun 28; 6:28693. Epub 2016 Jun 28
A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate
Nature Chemical Biology , April 25, 2016
A PHGDH inhibitor reveals coordination of serine synthesis and 1-carbon unit fate
Nat Chem Biol , 2016 Apr 25; 12(6):452-458. Epub 2016 Apr 25
Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers
Proc Natl Acad Sci U S A , 2016 Feb 1; 113(7):1778-1783. Epub 2016 Feb 1
An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation
Cancer Metab , 2015; 3:5. Epub 2015 Apr 29
NRF2 regulates serine biosynthesis in non-small cell lung cancer
Nat Genet , 2015/12/01; 47(12):1475-1481. Epub 2015 Oct 19
High level PHGDH expression in breast is predominantly associated with keratin 5‐positive cell lineage independently of malignancy
Mol Oncol , 2015 May 14; 9(8):1636-1654. Epub 2015 May 14
Cells deficient in base-excision repair reveal cancer hallmarks originating from adjustments to genetic instability
Nucleic Acids Res , 2015 Apr 20; 43(7):3667-3679. Epub 2015 Mar 23
p53 Protein-mediated Regulation of Phosphoglycerate Dehydrogenase (PHGDH) Is Crucial for the Apoptotic Response upon Serine Starvation
J Biol Chem , 2015/01/02; 290(1):457-466. Epub 2014 Nov 17
Serine starvation induces stress and p53-dependent metabolic remodelling in cancer cells.
Nature , 2013 Jan 24; 493(7433):542-6. Epub 2012 Dec 16
Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
PLoS Genet , 2012; 8(3):e1002573. Epub 2012 Mar 15
Serine starvation induces stress and p53 dependent metabolic remodeling in cancer cells
Nature , 2012 Dec 16; 493(7433):542-546. Epub 2012 Dec 16
Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.
Nature , 2011 Aug 18; 476(7360):346-50. Epub 2011 Aug 18
Reducing the serine availability complements the inhibition of the glutamine metabolism to block leukemia cell growth
Oncotarget , 1/01/01; 7(2):1765-1776. Epub 2015 Nov 28
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