Polyclonal Anti-PHF6 Antibody
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000025626 (98%)
Rat ENSRNOG00000002276 (98%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
PHF6 and JAK3 mutations cooperate to drive T-cell acute lymphoblastic leukemia progression
Leukemia , 2021 Aug 31; 36(2):370-382. Epub 2021 Aug 31
Loss of PHF6 leads to aberrant development of human neuron-like cells
Sci Rep , 2020 Nov 4; 10:19030. Epub 2020 Nov 4
Phf6 loss enhances HSC self-renewal driving tumor initiation and leukemia stem cell activity in T-ALL.
Cancer Discov , 2018 Dec 19; 9(3):436-451. Epub 2018 Dec 19
The Human Protein Atlas--a tool for pathology.
J Pathol , 2008 Dec; 216(4):387-93
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.