Enhanced Validation

Polyclonal Anti-PHF6 Antibody

PHD finger protein 6
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human PHF6
Alternative Gene Names
BFLS, BORJ, CENP-31, KIAA1823, MGC14797
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
PHD finger protein 6
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000025626 (98%)

Rat ENSRNOG00000002276 (98%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
PHD finger protein 6
Gene Name
Alternative Gene Names
BFLS, BORJ, CENP-31, KIAA1823, MGC14797
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000156531 on the Human Protein Atlas

Yuan S, Wang X, Hou S, Guo T, Lan Y, Yang S, Zhao F, Gao J, Wang Y, Chu Y, Shi J, Cheng T, Yuan W

PHF6 and JAK3 mutations cooperate to drive T-cell acute lymphoblastic leukemia progression

Leukemia , 2021 Aug 31; 36(2):370-382. Epub 2021 Aug 31

PubMed ID: 34465864 DOI: 10.1038/s41375-021-01392-1

Fliedner A, Gregor A, Ferrazzi F, Ekici AB, Sticht H, Zweier C

Loss of PHF6 leads to aberrant development of human neuron-like cells

Sci Rep , 2020 Nov 4; 10:19030. Epub 2020 Nov 4

PubMed ID: 33149206 DOI: 10.1038/s41598-020-75999-2

Wendorff AA, Quinn SA, Rashkovan M, Madubata CJ, Ambesi-Impiombato A, Litzow MR, Tallman MS, Paietta E, Paganin M, Basso G, Gastier-Foster JM, Loh ML, Rabadan R, Van Vlierberghe P, Ferrando AA

Phf6 loss enhances HSC self-renewal driving tumor initiation and leukemia stem cell activity in T-ALL.

Cancer Discov , 2018 Dec 19; 9(3):436-451. Epub 2018 Dec 19

PubMed ID: 30567843 DOI: 10.1158/2159-8290.CD-18-1005

Pontén F, Jirström K, Uhlen M

The Human Protein Atlas--a tool for pathology.

J Pathol , 2008 Dec; 216(4):387-93

PubMed ID: 18853439 DOI: 10.1002/path.2440

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Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Corresponding Antigens


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