Polyclonal Anti-PDK3 Antibody

pyruvate dehydrogenase kinase, isozyme 3
References (2)
Recommended Applications
Product Description
Polyclonal Antibody against Human PDK3
Open Datasheet
Price
$505.00
Product Number
HPA046583
Unit Size
Concentration
Lot dependent
Availability
7 in Stock
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[1 pictures]
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
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[1 pictures]
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
pyruvate dehydrogenase kinase, isozyme 3
Target Gene
PDK3
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
NKSPEDPQVLDNFLQVLIKVRNRHNDV
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000035232 (85%)

Rat ENSRNOG00000012513 (85%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for HPA046583 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
pyruvate dehydrogenase kinase, isozyme 3
Gene Name
PDK3
UniProt ID
Q15120
Gene (Ensembl)
ENSG00000067992
Entrez Gene ID
Entrez gene: 5165
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000067992 on the Human Protein Atlas

Nunes-Xavier CE, Mingo J, Emaldi M, Flem-Karlsen K, Mælandsmo GM, Fodstad Ø, Llarena R, López JI, Pulido R

Heterogeneous Expression and Subcellular Localization of Pyruvate Dehydrogenase Complex in Prostate Cancer

Front Oncol , 2022 May 25; 12:873516. Epub 2022 May 25

PubMed ID: 35692804 DOI: 10.3389/fonc.2022.873516

Did we miss your publication?

Have you published using HPA046583? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-PDK3 (HPA072492)
Corresponding Antigens
PrEST Antigen PDK3 (APrEST78897)

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