Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-PDIA3 HPA002645.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Characterization of Heparan Sulfate Proteoglycan-positive Recycling Endosomes Isolated from Glioma Cells
Cancer Genomics Proteomics , 2016 Nov 12; 13(6):443-452. Epub 2016 Oct 8
The dehydrogenase region of the NADPH oxidase component Nox2 acts as a protein disulfide isomerase (PDI) resembling PDIA3 with a role in the binding of the activator protein p67phox
Front Chem , 2015; 3:3. Epub 2015 Feb 4
Proteomics Based Identification of Cell Migration Related Proteins in HBV Expressing HepG2 Cells
PLoS One , 2014; 9(4):e95621. Epub 2014 Apr 24
Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling.
Nat Genet , 2014 Feb; 46(2):188-93. Epub 2013 Dec 15
The Involvement of SMILE/TMTC3 in Endoplasmic Reticulum Stress Response
PLoS One , 2011; 6(5):e19321. Epub 2011 May 16
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