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Enhanced Validation

Anti-PDIA3 Antibody

protein disulfide isomerase family A, member 3
References (3)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human PDIA3
Alternative Gene Names
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Open Datasheet
Price
$437.00
Product Number
HPA002645
Unit Size
100 µl
Concentration
Lot dependent
Availability
1 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using Anti-PDIA3 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDIA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target protein
protein disulfide isomerase family A, member 3
Target gene
PDIA3
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
HMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGP
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000027248 (95%)

Rat ENSRNOG00000015018 (95%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.4 µg/ml

Immunofluorescence in cell lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 1-4 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-PDIA3 HPA003230.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA002645 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.

Immunofluorescence in cell lines (ICC-IF)

All ICC-IF characterization data for HPA002645 on the Human Protein Atlas
Protein Name
protein disulfide isomerase family A, member 3
Gene Name
PDIA3
Alternative Gene Names
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
UniProt ID
P30101
Gene (Ensembl)
ENSG00000167004
Entrez Gene ID
Entrez gene: 2923
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000167004 on the Human Protein Atlas

Zou H, Wen C, Peng Z, Shao YY, Hu L, Li S, Li C, Zhou HH

P4HB and PDIA3 are associated with tumor progression and therapeutic outcome of diffuse gliomas

Oncol Rep , 2017 Dec 4; 39(2):501-510. Epub 2017 Dec 4

PubMed ID: 29207176 DOI: 10.3892/or.2017.6134

Kashyap MK, Harsha H, Renuse S, Pawar H, Sahasrabuddhe NA, Kim MS, Marimuthu A, Keerthikumar S, Muthusamy B, Kandasamy K, Subbannayya Y, Prasad TS, Mahmood R, Chaerkady R, Meltzer SJ, Kumar RV, Rustgi AK, Pandey A

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

Cancer Biol Ther , 2010 Oct 15; 10(8):796-810. Epub 2010 Oct 7

PubMed ID: 20686364 DOI: 10.4161/cbt.10.8.12914

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