Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000015018 (95%)
Mouse ENSMUSG00000027248 (95%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-PDIA3 HPA003230.
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
P4HB and PDIA3 are associated with tumor progression and therapeutic outcome of diffuse gliomas
Oncol Rep , 2017 Dec 4; 39(2):501-510. Epub 2017 Dec 4
SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome
Cancer Biol Ther , 2010 Oct 15; 10(8):796-810. Epub 2010 Oct 7
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