Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
MiR-21 over-expression and Programmed Cell Death 4 down-regulation features malignant pleural mesothelioma
Oncotarget , 2018 Apr 3; 9(25):17300-17308. Epub 2018 Apr 3
MicroRNA Profiles in Familial and Sporadic Medullary Thyroid Carcinoma: Preliminary Relationships with RET Status and Outcome
Thyroid , 2012 Sep; 22(9):890-896
Programmed cell death 4 nuclear loss and miR-21 or activated Akt overexpression in esophageal squamous cell carcinogenesis.
Dis Esophagus , 2012 Apr; 25(3):263-8. Epub 2011 Aug 24
PDCD4 nuclear loss inversely correlates with miR-21 levels in colon carcinogenesis.
Virchows Arch , 2011 Apr; 458(4):413-9. Epub 2011 Jan 29
Programmed cell death 4 (PDCD4) expression during multistep Barrett's carcinogenesis
J Clin Pathol , 2010 Aug; 63(8):692-696. Epub 2010 Aug 11
MicroRNA expression profiling of human metastatic cancers identifies cancer gene targets.
J Pathol , 2009 Oct; 219(2):214-21
Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics
Mol Cell Proteomics , 2009 Jul; 8(7):1612-1622. Epub 2009 Apr 7
From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Mol Cell Proteomics , 2006 Jun; 5(6):1072-81. Epub 2006 Mar 8
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not articipate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 50% of the product price as a refund, or 50% discount on your next vial purchase.