Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000029470 (83%)
Rat ENSRNOG00000001300 (77%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
ATP-mediated Events in Peritubular Cells Contribute to Sterile Testicular Inflammation
Sci Rep , 2018 Jan 23; 8:1431. Epub 2018 Jan 23
Microglia‐derived purines modulate mossy fibre synaptic transmission and plasticity through P2X4 and A1 receptors
Eur J Neurosci , 2016 Mar 1; 43(10):1366-1378. Epub 2016 Mar 1
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