Enhanced Validation

Polyclonal Anti-OTC Antibody

ornithine carbamoyltransferase
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human OTC
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
ornithine carbamoyltransferase
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000031173 (93%)

Rat ENSRNOG00000003370 (92%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:2500 - 1:5000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-OTC HPA000570.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
ornithine carbamoyltransferase
Gene Name
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000036473 on the Human Protein Atlas

Zabulica M, Srinivasan RC, Akcakaya P, Allegri G, Bestas B, Firth M, Hammarstedt C, Jakobsson T, Jakobsson T, Ellis E, Jorns C, Makris G, Scherer T, Rimann N, van Zuydam NR, Gramignoli R, Forslöw A, Engberg S, Maresca M, Rooyackers O, Thöny B, Häberle J, Rosen B, Strom SC

Correction of a urea cycle defect after ex vivo gene editing of human hepatocytes

Mol Ther , 2021 Jan 21; 29(5):1903-1917. Epub 2021 Jan 21

PubMed ID: 33484963 DOI: 10.1016/j.ymthe.2021.01.024

Ginn SL, Amaya AK, Liao SH, Zhu E, Cunningham SC, Lee M, Hallwirth CV, Logan GJ, Tay SS, Cesare AJ, Pickett HA, Grompe M, Dilworth K, Lisowski L, Alexander IE

Efficient in vivo editing of OTC-deficient patient-derived primary human hepatocytes

JHEP Rep , 2019 Dec 27; 2(1):100065. Epub 2019 Dec 27

PubMed ID: 32039406 DOI: 10.1016/j.jhepr.2019.100065

Herrero A, Prigent J, Lombard C, Rosseels V, Daujat-Chavanieu M, Breckpot K, Najimi M, Deblandre G, Sokal EM

Adult-Derived Human Liver Stem/Progenitor Cells Infused 3 Days Postsurgery Improve Liver Regeneration in a Mouse Model of Extended Hepatectomy

Cell Transplant , 2017 Feb 1; 26(2):351-364. Epub 2017 Feb 1

PubMed ID: 27657746 DOI: 10.3727/096368916X692960

Wu CC, Hsu CW, Chen CD, Yu CJ, Chang KP, Tai DI, Liu HP, Su WH, Chang YS, Yu JS

Candidate Serological Biomarkers for Cancer Identified from the Secretomes of 23 Cancer Cell Lines and the Human Protein Atlas

Mol Cell Proteomics , 2010 Jun; 9(6):1100-1117. Epub 2010 Feb 1

PubMed ID: 20124221 DOI: 10.1074/mcp.M900398-MCP200

Chaerkady R, Harsha HC, Nalli A, Gucek M, Vivekanandan P, Akhtar J, Cole RN, Simmers J, Schulick RD, Singh S, Torbenson M, Pandey A, Thuluvath PJ

A Quantitative Proteomic Approach for Identification of Potential Biomarkers in Hepatocellular Carcinoma

J Proteome Res , 2008 Oct; 7(10):4289-4298. Epub 2008 Aug 21

PubMed ID: 18715028 DOI: 10.1021/pr800197z

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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