Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Myosin VI-Dependent Actin Cages Encapsulate Parkin-Positive Damaged Mitochondria
Dev Cell , 2018 Feb 26; 44(4):484-499.e6
Disruption of macrophage pro-inflammatory cytokine release in Crohn's disease is associated with reduced optineurin expression in a subset of patients
Immunology , 2015 Jan; 144(1):45-55. Epub 2014 Dec 8
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