Polyclonal Anti-NSL1 Antibody

NSL1, MIS12 kinetochore complex component
References (1)
Recommended Applications
Product Description
Polyclonal Antibody against Human NSL1
Alternative Gene Names
C1orf48, DC8, DKFZP566O1646, MIS14
Open Datasheet
Price
$505.00
Product Number
HPA053721
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[1 pictures]
Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Show all images
[1 pictures]
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & nuclear speckles.

Additional Images

See additional ICC images using this antibody on the Human Protein Atlas
Target Protein
NSL1, MIS12 kinetochore complex component
Target Gene
NSL1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
RKILECVIKTIKAKQEILKQYHPVVHPLDLKYDPDPAPHMENLKCRGETVAKEISEAMKSLPALIEQGE
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000062510 (75%)

Rat ENSRNOG00000042286 (72%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA053721 on the Human Protein Atlas
Protein Name
NSL1, MIS12 kinetochore complex component
Gene Name
NSL1
Alternative Gene Names
C1orf48, DC8, DKFZP566O1646, MIS14
UniProt ID
Q96IY1
Gene (Ensembl)
ENSG00000117697
Entrez Gene ID
Entrez gene: 25936
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000117697 on the Human Protein Atlas

Did we miss your publication?

Have you published using HPA053721? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-NSL1 (HPA045761)
Corresponding Antigens
PrEST Antigen NSL1 (APrEST89701)

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