Enhanced Validation

Anti-NLK Antibody

nemo-like kinase
Recommended Applications
Recombinant expression validation in WB using target protein overexpression.
Product Description
Polyclonal Antibody against Human NLK
Product Number
Unit Size
100 µl
Lot dependent
On Demand
On Demand
Target protein
nemo-like kinase
Target gene
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000017376 (100%)

Rat ENSRNOG00000008704 (100%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
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Product Data Sheet
Western Blot (WB)

Recommended conditions:

Working concentration: 0.4 µg/ml

Immunofluorescence in cell lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 1-4 µg/ml

Western Blot (WB)

Recombinant expression validation in WB using target protein overexpression.

Protein Name
nemo-like kinase
Gene Name
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000087095 on the Human Protein Atlas

Suwei D, Liang Z, Zhimin L, Ruilei L, Yingying Z, Zhen L, Chunlei G, Zhangchao L, Yuanbo X, Jinyan Y, Gaofeng L, Xin S

NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin

J Hematol Oncol , 2015 Oct 26; 8:120. Epub 2015 Oct 26

PubMed ID: 26503334 DOI: 10.1186/s13045-015-0203-8

Zhang B, Li KY, Chen HY, Pan SD, Chen SF, Zhang WF, Xia CP, Jiang LC, Liu XB, Zhao FJ, Yuan DY, Wang LX, Wu YP, Liu SW

Lentivirus-based RNA Silencing of Nemo-like Kinase (NLK) Inhibits the CAL 27 Human Adenosquamos Carcinoma Cells Proliferation and Blocks G0/G1 Phase to S Phase

Int J Med Sci , 2013; 10(10):1301-1306. Epub 2013 Aug 8

PubMed ID: 23983589 DOI: 10.7150/ijms.6607

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Alternative Antibodies
Corresponding Antigens


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