Enhanced Validation

Anti-NFE2L3 Antibody

nuclear factor, erythroid 2-like 3
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human NFE2L3
Alternative Gene Names
Product Number
Unit Size
Lot dependent
8 in Stock
Ready to Ship
Target Protein
nuclear factor, erythroid 2-like 3
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000029832 (67%)

Rat ENSRNOG00000010886 (58%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
nuclear factor, erythroid 2-like 3
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000050344 on the Human Protein Atlas

Kobayashi A

Roles of NRF3 in the Hallmarks of Cancer: Proteasomal Inactivation of Tumor Suppressors

Cancers (Basel) , 2020 Sep 20; 12(9):2681. Epub 2020 Sep 20

PubMed ID: 32962187 DOI: 10.3390/cancers12092681

Wang H, Zhan M, Yang R, Shi Y, Liu Q, Wang J

Elevated expression of NFE2L3 predicts the poor prognosis of pancreatic cancer patients

Cell Cycle , 2018 Sep 22; 17(17):2164-2174. Epub 2018 Sep 22

PubMed ID: 30196752 DOI: 10.1080/15384101.2018.1520558

Wang C, Saji M, Justiniano SE, Yusof AM, Zhang X, Yu L, Fernández S, Wakely P Jr, La Perle K, Nakanishi H, Pohlman N, Ringel MD

RCAN1-4 is a thyroid cancer growth and metastasis suppressor

JCI Insight , 2017 Mar 9; 2(5):e90651. Epub 2017 Mar 9

PubMed ID: 28289712 DOI: 10.1172/jci.insight.90651

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Corresponding Antigens


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