Lane 2: Human cell line SK-MEL-30
Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-NES HPA007007.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
CT-2A neurospheres-derived high-grade glioma in mice: a new model to address tumor stem cells and immunosuppression
Biol Open , 2019 Sep 11; 8(9):bio044552. Epub 2019 Sep 11
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