Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-NES HPA026111.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Proteomic analysis of gemcitabine-resistant pancreatic cancer cells reveals that microtubule-associated protein 2 upregulation associates with taxane treatment
Ther Adv Med Oncol , 2019 May 10; 11:1758835919841233. Epub 2019 May 10
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15
Spatiotemporal expression pattern of neuroepithelial stem cell marker nestin suggests a role in dermal homeostasis, neovasculogenesis, and tumor stroma development: a study on embryonic and adult human skin.
J Am Acad Dermatol , 2010 Jul; 63(1):93-113. Epub 2009 Oct 27
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