Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000013916 (77%)
Mouse ENSMUSG00000022054 (74%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Plasma profiling reveals three proteins associated to amyotrophic lateral sclerosis
Ann Clin Transl Neurol , 2014 Aug; 1(8):544-553. Epub 2014 Jul 14
Affinity Proteomics Reveals Elevated Muscle Proteins in Plasma of Children with Cerebral Malaria
PLoS Pathog , 2014 Apr; 10(4):e1004038. Epub 2014 Apr 17
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