Anti-NECAB2 Antibody



Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000031837 (98%)
Rat ENSRNOG00000015084 (97%)
Recommended conditions:
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-NECAB2 HPA014144.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000103154 on the Human Protein Atlas
Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
Sci Rep , 2018 Jan 12; 8:603. Epub 2018 Jan 12
Neuronal calcium-binding proteins 1/2 localize to dorsal root ganglia and excitatory spinal neurons and are regulated by nerve injury
Proc Natl Acad Sci U S A , 2014 Mar 25; 111(12):E1149-E1158. Epub 2014 Mar 10
Did we miss your publication?
Have you published using HPA013998? Please let us know and we will be happy to include your reference on this page.
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.
Questions?
Our customer support and expert scientific support teams are here to help you succeed in your research.