Enhanced Validation

Polyclonal Anti-NECAB1 Antibody

N-terminal EF-hand calcium binding protein 1
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Product Description
Polyclonal Antibody against Human NECAB1
Alternative Gene Names
EFCBP1
Open Datasheet
Price
$505.00
Product Number
HPA025963
Unit Size
100 µl
Concentration
Lot dependent
Availability
5 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA025963 antibody. Corresponding NECAB1 RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis using Anti-NECAB1 antibody HPA025963 (A) shows similar pattern to independent antibody HPA023629 (B).
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[1 pictures]
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
N-terminal EF-hand calcium binding protein 1
Target Gene
NECAB1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
ASSQSGCLRISIQKLSNESRYMIYEFWENSSVWNSHLQTNYSKTFQRSNVDFLETPELTSTMLVPASWW
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000040536 (97%)

Rat ENSRNOG00000007256 (97%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibodies Anti-NECAB1 HPA031262, Anti-NECAB1 HPA023629.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA025963 on the Human Protein Atlas
Western Blot (WB)

Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA025963 on the Human Protein Atlas
Protein Name
N-terminal EF-hand calcium binding protein 1
Gene Name
NECAB1
Alternative Gene Names
EFCBP1
UniProt ID
Q8N987
Gene (Ensembl)
ENSG00000123119
Entrez Gene ID
Entrez gene: 64168
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000123119 on the Human Protein Atlas

Udagawa H, Funahashi N, Nishimura W, Uebanso T, Kawaguchi M, Asahi R, Nakajima S, Nammo T, Hiramoto M, Yasuda K

Glucocorticoid receptor-NECAB1 axis can negatively regulate insulin secretion in pancreatic β-cells

Sci Rep , 2023 Oct 20; 13:17958. Epub 2023 Oct 20

PubMed ID: 37863964 DOI: 10.1038/s41598-023-44324-y

Did we miss your publication?

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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