Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
MicroRNA suppression of stress-responsive NDRG2 during dexamethasone treatment in skeletal muscle cells
BMC Mol Cell Biol , 2019 May 28; 20:12. Epub 2019 May 28
Abundant NDRG2 Expression Is Associated with Aggressiveness and Unfavorable Patients’ Outcome in Basal-Like Breast Cancer
PLoS One , 2016 Jul 11; 11(7):e0159073. Epub 2016 Jul 11
NDRG2 promotes myoblast proliferation and caspase 3/7 activities during differentiation, and attenuates hydrogen peroxide – But not palmitate-induced toxicity
FEBS Open Bio , 2015; 5:668-681. Epub 2015 Aug 7
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
NDRG4 Is Required for Cell Cycle Progression and Survival in Glioblastoma Cells
J Biol Chem , 2009 Sep 11; 284(37):25160-25169. Epub 2009 Jul 10
Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics
Mol Cell Proteomics , 2009 Jul; 8(7):1612-1622. Epub 2009 Apr 7
Frequent promoter hypermethylation and transcriptional downregulation of the NDRG2 gene at 14q11.2 in primary glioblastoma.
Int J Cancer , 2008 Nov 1; 123(9):2080-6
Contribution from Marlena Rohm, Ruhr University Bochum — May 07, 2018
We have successfully tested the Anti-NDRG2 antibody (HPA002896) by Western blot in mouse spinal cord lysates.
Loaded Sample: Protein isolated out of spinal cord, 30 mg protein
Primary Antibody: Anti-NDRG2 antibody (HPA002896) 1:750 in TBS – over night, 4°C, shaking
Secondary Antibody: Anti-rabbit HRP 1:200 in TBS – 1h, room temp, shaking
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