Enhanced Validation

Anti-MYO1C Antibody

myosin IC
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human MYO1C
Alternative Gene Names
myr2
Price
$445.00
Product Number
HPA001768
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
myosin IC
Target Gene
MYO1C
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
GEEETLRRLGLERNPQSYLYLVKGQCAKVSSINDKSDWKVVRKALTVIDFTEDEVEDLLSIVASVLHLGNIHFAANEESNAQVTTENQLKYLTRLLSVEGSTLREALTHRKIIAKGEELLSPLNLEQAAYARDALAKAVYSRTFTWLV
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000017774 (96%)

Rat ENSRNOG00000004072 (96%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
myosin IC
Gene Name
MYO1C
Alternative Gene Names
myr2
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000197879 on the Human Protein Atlas

Nevzorov I, Sidorenko E, Wang W, Zhao H, Vartiainen MK

Myosin‐1C uses a novel phosphoinositide‐dependent pathway for nuclear localization

EMBO Rep , 2018 Jan 12; 19(2):290-304. Epub 2018 Jan 12

PubMed ID: 29330316 DOI: 10.15252/embr.201744296

Lehtimäki JI, Fenix AM, Kotila TM, Balistreri G, Paavolainen L, Varjosalo M, Burnette DT, Lappalainen P

UNC-45a promotes myosin folding and stress fiber assembly

J Cell Biol , 2017 Dec 4; 216(12):4053-4072

PubMed ID: 29055011 DOI: 10.1083/jcb.201703107

Visuttijai K, Pettersson J, Mehrbani Azar Y, van den Bout I, Örndal C, Marcickiewicz J, Nilsson S, Hörnquist M, Olsson B, Ejeskär K, Behboudi A

Lowered Expression of Tumor Suppressor Candidate MYO1C Stimulates Cell Proliferation, Suppresses Cell Adhesion and Activates AKT

PLoS One , 2016 Oct 7; 11(10):e0164063. Epub 2016 Oct 7

PubMed ID: 27716847 DOI: 10.1371/journal.pone.0164063

Hedberg Oldfors C, Dios DG, Linder A, Visuttijai K, Samuelson E, Karlsson S, Nilsson S, Behboudi A

Analysis of an independent tumor suppressor locus telomeric to Tp53 suggested Inpp5k and Myo1c as novel tumor suppressor gene candidates in this region

BMC Genet , 2015 Jul 14; 16:80. Epub 2015 Jul 14

PubMed ID: 26170120 DOI: 10.1186/s12863-015-0238-4

Brandstaetter H, Kishi-Itakura C, Tumbarello DA, Manstein DJ, Buss F

Loss of functional MYO1C/myosin 1c, a motor protein involved in lipid raft trafficking, disrupts autophagosome-lysosome fusion

Autophagy , 2014 Dec 31; 10(12):2310-2323. Epub 2015 Jan 28

PubMed ID: 25551774 DOI: 10.4161/15548627.2014.984272

Tiwari A, Jung JJ, Inamdar SM, Nihalani D, Choudhury A

The myosin motor Myo1c is required for VEGFR2 delivery to the cell surface and for angiogenic signaling

Am J Physiol Heart Circ Physiol , 2013 Mar 1; 304(5):H687-H696. Epub 2012 Dec 21

PubMed ID: 23262137 DOI: 10.1152/ajpheart.00744.2012

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Alternative Antibodies
Corresponding Antigens

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