Enhanced Validation

Polyclonal Anti-MTDH Antibody

metadherin
References (3)
Recommended Applications
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Product Description
Polyclonal Antibody against Human MTDH
Alternative Gene Names
3D3, AEG-1, LYRIC
Open Datasheet
Price
$505.00
Product Number
HPA010932
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[1 pictures]
Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.
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[2 pictures]
Western blot analysis using Anti-MTDH antibody HPA010932 (A) shows similar pattern to independent antibody HPA015104 (B).
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[1 pictures]
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
metadherin
Target Gene
MTDH
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
RKTEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQD
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000022255 (93%)

Rat ENSRNOG00000006870 (90%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for HPA010932 on the Human Protein Atlas
Western Blot (WB)

Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA010932 on the Human Protein Atlas
Protein Name
metadherin
Gene Name
MTDH
Alternative Gene Names
3D3, AEG-1, LYRIC
UniProt ID
Q86UE4
Gene (Ensembl)
ENSG00000147649
Entrez Gene ID
Entrez gene: 92140
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000147649 on the Human Protein Atlas

Bi J, Yang S, Li L, Dai Q, Borcherding N, Wagner BA, Buettner GR, Spitz DR, Leslie KK, Zhang J, Meng X

Metadherin enhances vulnerability of cancer cells to ferroptosis

Cell Death Dis , 2019 Sep 17; 10(10):682. Epub 2019 Sep 17

PubMed ID: 31527591 DOI: 10.1038/s41419-019-1897-2

LIU B, WU Y, PENG D

Astrocyte elevated gene-1 regulates osteosarcoma cell invasion and chemoresistance via endothelin-1/endothelin A receptor signaling

Oncol Lett , 2013 Feb; 5(2):505-510. Epub 2012 Dec 3

PubMed ID: 23420812 DOI: 10.3892/ol.2012.1056

Did we miss your publication?

Have you published using HPA010932? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-MTDH (AMAb90762)
Anti-MTDH (AMAb90763)
Anti-MTDH (HPA015104)
Corresponding Antigens
PrEST Antigen MTDH (APrEST72056)

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