Enhanced Validation

Polyclonal Anti-MKI67 Antibody

marker of proliferation Ki-67
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human MKI67
Alternative Gene Names
MIB-, PPP1R105
Product Number
Unit Size
Lot dependent
On Demand
On Demand
Target Protein
marker of proliferation Ki-67
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000028137 (68%)

Mouse ENSMUSG00000031004 (68%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
marker of proliferation Ki-67
Gene Name
Alternative Gene Names
MIB-, PPP1R105
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000148773 on the Human Protein Atlas

Garavaglia B, Vallino L, Ferraresi A, Esposito A, Salwa A, Vidoni C, Gentilli S, Isidoro C

Butyrate Inhibits Colorectal Cancer Cell Proliferation through Autophagy Degradation of β-Catenin Regardless of APC and β-Catenin Mutational Status

Biomedicines , 2022 May 13; 10(5):1131. Epub 2022 May 13

PubMed ID: 35625868 DOI: 10.3390/biomedicines10051131

Secomandi E, Salwa A, Vidoni C, Ferraresi A, Follo C, Isidoro C

High Expression of the Lysosomal Protease Cathepsin D Confers Better Prognosis in Neuroblastoma Patients by Contrasting EGF-Induced Neuroblastoma Cell Growth

Int J Mol Sci , 2022 Apr 26; 23(9):4782. Epub 2022 Apr 26

PubMed ID: 35563171 DOI: 10.3390/ijms23094782

Esposito A, Ferraresi A, Salwa A, Vidoni C, Dhanasekaran DN, Isidoro C

Resveratrol Contrasts IL-6 Pro-Growth Effects and Promotes Autophagy-Mediated Cancer Cell Dormancy in 3D Ovarian Cancer: Role of miR-1305 and of Its Target ARH-I

Cancers (Basel) , 2022 Apr 25; 14(9):2142. Epub 2022 Apr 25

PubMed ID: 35565270 DOI: 10.3390/cancers14092142

Geread RS, Sivanandarajah A, Brouwer ER, Wood GA, Androutsos D, Faragalla H, Khademi A

piNET–An Automated Proliferation Index Calculator Framework for Ki67 Breast Cancer Images

Cancers (Basel) , 2020 Dec 22; 13(1):11. Epub 2020 Dec 22

PubMed ID: 33375043 DOI: 10.3390/cancers13010011

Dusart P, Fagerberg L, Perisic L, Civelek M, Struck E, Hedin U, Uhlén M, Trégouët DA, Renné T, Odeberg J, Butler LM

A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein

Sci Rep , 2018 Oct 2; 8:14668. Epub 2018 Oct 2

PubMed ID: 30279450 DOI: 10.1038/s41598-018-32859-4

Roca H, Craig MJ, Ying C, Varsos ZS, Czarnieski P, Alva AS, Hernandez J, Fuller D, Daignault S, Healy PN, Pienta KJ

IL-4 induces proliferation in prostate cancer PC3 cells under nutrient-depletion stress through the activation of the JNK-pathway and survivin upregulation

J Cell Biochem , 2012 May; 113(5):1569-1580

PubMed ID: 22174091 DOI: 10.1002/jcb.24025

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