Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000033134 (99%)
Mouse ENSMUSG00000005583 (97%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Intercalated Cushion Cells Within The Cardiac Outflow Tract Are Derived from the Myocardial Troponin T Type 2 (Tnnt2) Cre Lineage
Dev Dyn , 2018 Jul 1; 247(8):1005-1017. Epub 2018 Jul 1
Mef2c Regulates Transcription of the Extracellular Matrix Protein Cartilage Link Protein 1 in the Developing Murine Heart
PLoS One , 2013; 8(2):e57073. Epub 2013 Feb 26
Evolutionary Conservation of Nkx2.5 Autoregulation in the Second Heart Field
Dev Biol , 2013 Feb 1; 374(1):198-209. Epub 2012 Nov 17
Differential expression of cartilage and bone-related proteins in pediatric and adult diseased aortic valves
J Mol Cell Cardiol , 2011 Mar; 50(3):561-569. Epub 2010 Dec 14
MicroRNA-21 dysregulates the expression of MEF2C in neurons in monkey and human SIV/HIV neurological disease
Cell Death Dis , 2010; 1(9):e77-. Epub 2010 Sep 23
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