Enhanced Validation

Polyclonal Anti-MCAM Antibody

melanoma cell adhesion molecule
References (4)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human MCAM
Alternative Gene Names
CD146, MUC18
Open Datasheet
Price
$505.00
Product Number
HPA008848
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[5 pictures]
Immunohistochemistry analysis in human placenta and liver tissues using HPA008848 antibody. Corresponding MCAM RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Western blot analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-MCAM antibody. Corresponding MCAM RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
melanoma cell adhesion molecule
Target Gene
MCAM
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
LNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLE
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000032135 (75%)

Rat ENSRNOG00000007726 (73%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA008848 on the Human Protein Atlas
Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Protein Name
melanoma cell adhesion molecule
Gene Name
MCAM
Alternative Gene Names
CD146, MUC18
UniProt ID
P43121
Gene (Ensembl)
ENSG00000076706
Entrez Gene ID
Entrez gene: 4162
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000076706 on the Human Protein Atlas

Akbar M, McLean M, Garcia-Melchor E, Crowe LA, McMillan P, Fazzi UG, Martin D, Arthur A, Reilly JH, McInnes IB, Millar NL

Fibroblast activation and inflammation in frozen shoulder

PLoS One , 2019 Apr 23; 14(4):e0215301. Epub 2019 Apr 23

PubMed ID: 31013287 DOI: 10.1371/journal.pone.0215301

Ferguson HJ, Wragg JW, Ward S, Heath VL, Ismail T, Bicknell R

Glutamate dependent NMDA receptor 2D is a novel angiogenic tumour endothelial marker in colorectal cancer

Oncotarget , 2016 Mar 1; 7(15):20440-20454. Epub 2016 Mar 1

PubMed ID: 26943033 DOI: 10.18632/oncotarget.7812

Wragg JW, Finnity JP, Anderson JA, Ferguson HJ, Porfiri E, Bhatt RI, Murray PG, Heath VL, Bicknell R

MCAM and LAMA4 are highly enriched in tumor blood vessels of renal cell carcinoma and predict patient outcome

Cancer Res , 2016 Feb 26; 76(8):2314-2326. Epub 2016 Feb 26

PubMed ID: 26921326 DOI: 10.1158/0008-5472.CAN-15-1364

Did we miss your publication?

Have you published using HPA008848? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Alternative Antibodies
Anti-MCAM (AMAb91815)
Corresponding Antigens
PrEST Antigen MCAM (APrEST71658)

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch