Enhanced Validation

Anti-LRG1 Antibody

leucine-rich alpha-2-glycoprotein 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human LRG1
Alternative Gene Names
LRG
Price
$450.00
Product Number
HPA001888
Unit Size
Concentration
Lot dependent
Availability
On Demand
Ships
On Demand
Target Protein
leucine-rich alpha-2-glycoprotein 1
Target Gene
LRG1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
TLSPKDCQVFRSDHGSSISCQPPAEIPGYLPADTVHLAVEFFNLTHLPANLLQGASKLQELHLSSNGLESLSPEFLRPVPQLRVLDLTRNALTGLPPGLFQASATLDTLVLKENQLEVLEVSWLHGLKALGHLDLSG
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000037095 (63%)

Rat ENSRNOG00000049918 (61%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
leucine-rich alpha-2-glycoprotein 1
Gene Name
LRG1
Alternative Gene Names
LRG
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000171236 on the Human Protein Atlas

Fujimoto M, Matsumoto T, Serada S, Tsujimura Y, Hashimoto S, Yasutomi Y, Naka T

Leucine-rich alpha 2 glycoprotein is a new marker for active disease of tuberculosis

Sci Rep , 2020 Feb 25; 10:3384. Epub 2020 Feb 25

PubMed ID: 32099022 DOI: 10.1038/s41598-020-60450-3

Zhang Q, Huang R, Tang Q, Yu Y, Huang Q, Chen Y, Wang G, Wang X

Leucine-rich alpha-2-glycoprotein-1 is up-regulated in colorectal cancer and is a tumor promoter

Onco Targets Ther , 2018 May 11; 11:2745-2752. Epub 2018 May 11

PubMed ID: 29785123 DOI: 10.2147/OTT.S153375

Yamamoto M, Takahashi T, Serada S, Sugase T, Tanaka K, Miyazaki Y, Makino T, Kurokawa Y, Yamasaki M, Nakajima K, Takiguchi S, Naka T, Mori M, Doki Y

Overexpression of leucine‐rich α2‐glycoprotein‐1 is a prognostic marker and enhances tumor migration in gastric cancer

Cancer Sci , 2017 Oct 2; 108(10):2052-2060. Epub 2017 Oct 2

PubMed ID: 28746773 DOI: 10.1111/cas.13329

Zhou Y, Zhang X, Zhang J, Fang J, Ge Z, Li X

LRG1 promotes proliferation and inhibits apoptosis in colorectal cancer cells via RUNX1 activation

PLoS One , 2017 Apr 4; 12(4):e0175122. Epub 2017 Apr 4

PubMed ID: 28376129 DOI: 10.1371/journal.pone.0175122

Honda H, Fujimoto M, Miyamoto S, Ishikawa N, Serada S, Hattori N, Nomura S, Kohno N, Yokoyama A, Naka T

Sputum Leucine-Rich Alpha-2 Glycoprotein as a Marker of Airway Inflammation in Asthma

PLoS One , 2016 Sep 9; 11(9):e0162672. Epub 2016 Sep 9

PubMed ID: 27611322 DOI: 10.1371/journal.pone.0162672

Lindén M, Segersten U, Runeson M, Wester K, Busch C, Pettersson U, Lind SB, Malmström PU

Tumour expression of bladder cancer-associated urinary proteins.

BJU Int , 2013 Aug; 112(3):407-15. Epub 2013 Mar 7

PubMed ID: 23470167 DOI: 10.1111/j.1464-410X.2012.11653.x

Wang X, Abraham S, McKenzie JA, Jeffs N, Swire M, Tripathi VB, Luhmann UF, Lange CA, Zhai Z, Arthur HM, Bainbridge J, Moss SE, Greenwood J

LRG1 promotes angiogenesis by modulating endothelial TGFß signalling

Nature , 2013 Jul 18; 499(7458):10.1038/nature1

PubMed ID: 23868260 DOI: 10.1038/nature12345

Wang X, Abraham S, McKenzie JA, Jeffs N, Swire M, Tripathi VB, Luhmann UF, Lange CA, Zhai Z, Arthur HM, Bainbridge J, Moss SE, Greenwood J

LRG1 promotes angiogenesis by modulating endothelial TGFß signalling

Nature , 2013 Jul 18; 499(7458):10.1038/nature12345

PubMed ID: 23868260 DOI: 10.1038/nature12345

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Alternative Antibodies
Corresponding Antigens

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