Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg
Sci Rep , 2019 Jun 11; 9:8461. Epub 2019 Jun 11
Repair of nuclear ruptures requires barrier-to-autointegration factor
J Cell Biol , 2019 May 30; 218(7):2136-2149. Epub 2019 May 30
LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells
Proc Natl Acad Sci U S A , 2017 Feb 27; 114(11):E2166-E2175. Epub 2017 Feb 27
Contribution from Dr. Irina Solovei, University of Munich (LMU) — November 11, 2014
We have successfully tested the Anti-LEMD2 antibody (HPA017340) in mouse tissues, both cultured cells and cryosections, using immunofluorescence.
A. B. C.
Confocal images showing nuclear membranous positivity in mouse tissues (A and B) and cultured mouse myocytes (C) after staining with Anti-LEMD2 Antibody (HPA017340).
Antibody staining is shown in green. Secondary antibody was donkey-anti-rabbit conjugated to DyLight 488 (Jackson Immuno Research, 711-485-152); nuclei were counterstained with DAPI. The images are single optical sections acquired using Leica SP5 confocal microscope.
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