Polyclonal Anti-LEMD2 Antibody
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000044857 (92%)
Rat ENSRNOG00000025864 (90%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Mechanisms of A-Type Lamin Targeting to Nuclear Ruptures Are Disrupted in LMNA- and BANF1-Associated Progerias
Cells , 2022 Mar 2; 11(5):865. Epub 2022 Mar 2
CDK1 controls CHMP7-dependent nuclear envelope reformation
eLife , 2021 Jul 21; 10:e59999. Epub 2021 Jul 21
The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization
Nat Commun , 2021 Jan 29; 12:690. Epub 2021 Jan 29
SATB2‐LEMD2 interaction links nuclear shape plasticity to regulation of cognition‐related genes
EMBO J , 2020 Dec 15; 40(3):e103701. Epub 2020 Dec 15
BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins
Mol Biol Cell , 2020 Jul 15; 31(15):1551-1560
LEM2 phase separation governs ESCRT-mediated nuclear envelope reformation
Nature , 2020 Apr 29; 582(7810):115-118. Epub 2020 Apr 29
Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg
Sci Rep , 2019 Jun 11; 9:8461. Epub 2019 Jun 11
Repair of nuclear ruptures requires barrier-to-autointegration factor
J Cell Biol , 2019 May 30; 218(7):2136-2149. Epub 2019 May 30
LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells
Proc Natl Acad Sci U S A , 2017 Feb 27; 114(11):E2166-E2175. Epub 2017 Feb 27
Contribution from Dr. Irina Solovei, University of Munich (LMU) — February 22, 2016
We have successfully tested the Anti-LEMD2 antibody (HPA017340) in mouse tissues, both cultured cells and cryosections, using immunofluorescence.
A. B. C.
Confocal images showing nuclear membranous positivity in mouse tissues (A and B) and cultured mouse myocytes (C) after staining with Anti-LEMD2 Antibody (HPA017340).
Antibody staining is shown in green. Secondary antibody was donkey-anti-rabbit conjugated to DyLight 488 (Jackson Immuno Research, 711-485-152); nuclei were counterstained with DAPI. The images are single optical sections acquired using Leica SP5 confocal microscope.
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.