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Anti-KMT2D Antibody

lysine (K)-specific methyltransferase 2D
References (6)
Recommended Applications
Product Description
Polyclonal Antibody against Human KMT2D
Alternative Gene Names
ALR, CAGL114, MLL2, MLL4, TNRC21
Open Datasheet
Price
$450.00
Product Number
HPA035977
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
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[1 pictures]
Immunofluorescent staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
lysine (K)-specific methyltransferase 2D
Target Gene
KMT2D
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
AQLADTLFSKGLGPWDPPDNLAETQKPEQSSLVPGHLDQVNGQVVPEASQLSIKQEPREEPCALGAQSVKREANGEPIGAPGTSNHLLLAGPRSEAGHLLLQKLLRAKNVQLSTGRGSEGLRAEINGHI
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000048154 (90%)

Rat ENSRNOG00000061499 (84%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Protein Name
lysine (K)-specific methyltransferase 2D
Gene Name
KMT2D
Alternative Gene Names
ALR, CAGL114, MLL2, MLL4, TNRC21
UniProt ID
O14686
Gene (Ensembl)
ENSG00000167548
Entrez Gene ID
Entrez gene: 8085
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000167548 on the Human Protein Atlas

Toska E, Castel P, Chhangawala S, Arruabarrena-Aristorena A, Chan C, Hristidis VC, Cocco E, Sallaku M, Xu G, Park J, Minuesa G, Shifman SG, Socci ND, Koche R, Leslie CS, Scaltriti M, Baselga J

PI3K Inhibition Activates SGK1 via a Feedback Loop to Promote Chromatin-Based Regulation of ER-Dependent Gene Expression

Cell Rep , 2019 Apr 2; 27(1):294-306.e5

PubMed ID: 30943409 DOI: 10.1016/j.celrep.2019.02.111

Hillman RT, Celestino J, Terranova C, Beird HC, Gumbs C, Little L, Nguyen T, Thornton R, Tippen S, Zhang J, Lu KH, Gershenson DM, Rai K, Broaddus RR, Futreal PA

KMT2D/MLL2 inactivation is associated with recurrence in adult-type granulosa cell tumors of the ovary

Nat Commun , 2018 Jun 27; 9:2496. Epub 2018 Jun 27

PubMed ID: 29950560 DOI: 10.1038/s41467-018-04950-x

Lin-Shiao E, Lan Y, Coradin M, Anderson A, Donahue G, Simpson CL, Sen P, Saffie R, Busino L, Garcia BA, Berger SL, Capell BC

KMT2D regulates p63 target enhancers to coordinate epithelial homeostasis

Genes Dev , 2018 Jan 15; 32(2):181-193

PubMed ID: 29440247 DOI: 10.1101/gad.306241.117

Zhang J, Dominguez-Sola D, Hussein S, Lee JE, Holmes AB, Bansal M, Vlasevska S, Mo T, Tang H, Basso K, Ge K, Dalla-Favera R, Pasqualucci L

Disruption of KMT2D perturbs germinal center B cell development and promotes lymphomagenesis

Nat Med , 2015 Sep 14; 21(10):1190-1198. Epub 2015 Sep 14

PubMed ID: 26366712 DOI: 10.1038/nm.3940

Dubuc AM, Remke M, Korshunov A, Northcott PA, Zhan SH, Mendez-Lago M, Kool M, Jones DT, Unterberger A, Morrissy AS, Shih D, Peacock J, Ramaswamy V, Rolider A, Wang X, Witt H, Hielscher T, Hawkins C, Vibhakar R, Croul S, Rutka JT, Weiss WA, Jones SJ, Eberhart CG, Marra MA, Pfister SM, Taylor MD

Aberrant patterns of H3K4 and H3K27 histone lysine methylation occur across subgroups in medulloblastoma

Acta Neuropathol , 2013 Mar; 125(3):373-384. Epub 2012 Nov 25

PubMed ID: 23184418 DOI: 10.1007/s00401-012-1070-9

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Alternative Antibodies
Anti-KMT2D (AMAb91368)
Corresponding Antigens
PrEST Antigen KMT2D (APrEST86867)

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