Enhanced Validation

Anti-KLF4 Antibody

Kruppel-like factor 4 (gut)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human KLF4
Alternative Gene Names
EZF, GKLF
Price
$450.00
Product Number
HPA002926
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
Kruppel-like factor 4 (gut)
Target Gene
KLF4
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
ETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSP
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000016299 (89%)

Mouse ENSMUSG00000003032 (89%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Recombinant expression validation in WB using target protein overexpression.

Protein Name
Kruppel-like factor 4 (gut)
Gene Name
KLF4
Alternative Gene Names
EZF, GKLF
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000136826 on the Human Protein Atlas

Guidry JT, Myers JE, Bienkowska-Haba M, Songock WK, Ma X, Shi M, Nathan CO, Bodily JM, Sapp MJ, Scott RS

Inhibition of Epstein-Barr Virus Replication in Human Papillomavirus-Immortalized Keratinocytes

J Virol , 2019 Jan 4; 93(2):e01216-18. Epub 2019 Jan 4

PubMed ID: 30381489 DOI: 10.1128/JVI.01216-18

Murgai M, Ju W, Eason M, Kline J, Beury D, Kaczanowska S, Miettinen MM, Kruhlak M, Lei H, Shern JF, Cherepanova OA, Owens GK, Kaplan RN

KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis

Nat Med , 2017 Sep 18; 23(10):1176-1190. Epub 2017 Sep 18

PubMed ID: 28920957 DOI: 10.1038/nm.4400

Feng J, Jing J, Li J, Zhao H, Punj V, Zhang T, Xu J, Chai Y

BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice

Development , 2017 Jul 15; 144(14):2560-2569

PubMed ID: 28576771 DOI: 10.1242/dev.150136

Zimmerlin L, Park TS, Huo JS, Verma K, Pather SR, Talbot CC Jr, Agarwal J, Steppan D, Zhang YW, Considine M, Guo H, Zhong X, Gutierrez C, Cope L, Canto-Soler MV, Friedman AD, Baylin SB, Zambidis ET

Tankyrase inhibition promotes a stable human naïve pluripotent state with improved functionality

Development , 2016 Dec 1; 143(23):4368-4380

PubMed ID: 27660325 DOI: 10.1242/dev.138982

Nawandar DM, Wang A, Makielski K, Lee D, Ma S, Barlow E, Reusch J, Jiang R, Wille CK, Greenspan D, Greenspan JS, Mertz JE, Hutt-Fletcher L, Johannsen EC, Lambert PF, Kenney SC

Differentiation-Dependent KLF4 Expression Promotes Lytic Epstein-Barr Virus Infection in Epithelial Cells

PLoS Pathog , 2015 Oct; 11(10):e1005195. Epub 2015 Oct 2

PubMed ID: 26431332 DOI: 10.1371/journal.ppat.1005195

Boxer LD, Barajas B, Tao S, Zhang J, Khavari PA

ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes

Genes Dev , 2014 Sep 15; 28(18):2013-2026

PubMed ID: 25228645 DOI: 10.1101/gad.246579.114

Hale AT, Tian H, Anih E, Recio FO III, Shatat MA, Johnson T, Liao X, Ramirez-Bergeron DL, Proweller A, Ishikawa M, Hamik A

Endothelial Krüppel-like Factor 4 Regulates Angiogenesis and the Notch Signaling Pathway

J Biol Chem , 2014 Apr 25; 289(17):12016-12028. Epub 2014 Mar 5

PubMed ID: 24599951 DOI: 10.1074/jbc.M113.530956

Elsir T, Edqvist PH, Carlson J, Ribom D, Bergqvist M, Ekman S, Popova SN, Alafuzoff I, Ponten F, Nistér M, Smits A

A study of embryonic stem cell-related proteins in human astrocytomas: identification of Nanog as a predictor of survival.

Int J Cancer , 2014 Mar 1; 134(5):1123-31. Epub 2013 Sep 16

PubMed ID: 24037901 DOI: 10.1002/ijc.28441

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Corresponding Antigens

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