Enhanced Validation

Anti-KDM5B Antibody

lysine (K)-specific demethylase 5B
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human KDM5B
Alternative Gene Names
CT31, JARID1B, PLU-1, PPP1R98, RBBP2H1A
Price
$437.00
Product Number
HPA027179
Unit Size
100 µl
Concentration
Lot dependent
Availability
3 in Stock
Ships
Ready to Ship
Target protein
lysine (K)-specific demethylase 5B
Target gene
KDM5B
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
DGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSE
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000060544 (91%)

Mouse ENSMUSG00000042207 (86%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
lysine (K)-specific demethylase 5B
Gene Name
KDM5B
Alternative Gene Names
CT31, JARID1B, PLU-1, PPP1R98, RBBP2H1A
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000117139 on the Human Protein Atlas

Shigekawa Y, Hayami S, Ueno M, Miyamoto A, Suzaki N, Kawai M, Hirono S, Okada KI, Hamamoto R, Yamaue H

Overexpression of KDM5B/JARID1B is associated with poor prognosis in hepatocellular carcinoma

Oncotarget , 2018 Sep 28; 9(76):34320-34335. Epub 2018 Sep 28

PubMed ID: 30344945 DOI: 10.18632/oncotarget.26144

Wu L, Cao J, Cai WL, Lang SM, Horton JR, Jansen DJ, Liu ZZ, Chen JF, Zhang M, Mott BT, Pohida K, Rai G, Kales SC, Henderson MJ, Hu X, Jadhav A, Maloney DJ, Simeonov A, Zhu S, Iwasaki A, Hall MD, Cheng X, Shadel GS, Yan Q

KDM5 histone demethylases repress immune response via suppression of STING

PLoS Biol , 2018 Aug 6; 16(8):e2006134. Epub 2018 Aug 6

PubMed ID: 30080846 DOI: 10.1371/journal.pbio.2006134

vel Szic KS, Declerck K, Crans RA, Diddens J, Scherf DB, Gerhäuser C, Berghe WV

Epigenetic silencing of triple negative breast cancer hallmarks by Withaferin A

Oncotarget , 2017 Apr 13; 8(25):40434-40453. Epub 2017 Apr 13

PubMed ID: 28467815 DOI: 10.18632/oncotarget.17107

Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q

An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting

Nucleic Acids Res , 2016 Jul 25; 44(19):e149. Epub 2016 Jul 25

PubMed ID: 27458201 DOI: 10.1093/nar/gkw660

Gale M, Sayegh J, Cao J, Norcia M, Gareiss P, Hoyer D, Merkel JS, Yan Q

Screen-identified selective inhibitor of lysine demethylase 5A blocks cancer cell growth and drug resistance

Oncotarget , 2016 May 21; 7(26):39931-39944. Epub 2016 May 21

PubMed ID: 27224921 DOI: 10.18632/oncotarget.9539

Wang L, Mao Y, Du G, He C, Han S

Overexpression of JARID1B is associated with poor prognosis and chemotherapy resistance in epithelial ovarian cancer

Tumour Biol , 2015 Apr; 36(4):2465-2472. Epub 2015 Feb 8

PubMed ID: 25663457 DOI: 10.1007/s13277-014-2859-z

Nishibuchi G, Shibata Y, Hayakawa T, Hayakawa N, Ohtani Y, Sinmyozu K, Tagami H, Nakayama JI

Physical and Functional Interactions between the Histone H3K4 Demethylase KDM5A and the Nucleosome Remodeling and Deacetylase (NuRD) Complex

J Biol Chem , 2014 Oct 17; 289(42):28956-28970. Epub 2014 Sep 4

PubMed ID: 25190814 DOI: 10.1074/jbc.M114.573725

Zou MR, Cao J, Liu Z, Huh SJ, Polyak K, Yan Q

Histone Demethylase Jumonji AT-rich Interactive Domain 1B (JARID1B) Controls Mammary Gland Development by Regulating Key Developmental and Lineage Specification Genes

J Biol Chem , 2014 Jun 20; 289(25):17620-17633. Epub 2014 May 6

PubMed ID: 24802759 DOI: 10.1074/jbc.M114.570853

Yamamoto S, Wu Z, Russnes HG, Takagi S, Peluffo G, Vaske C, Zhao X, Vollan HK, Maruyama R, Ekram MB, Sun H, Kim JH, Carver K, Zucca M, Feng J, Almendro V, Bessarabova M, Rueda OM, Nikolsky Y, Caldas C, Liu XS, Polyak K

JARID1B is a luminal lineage-driving oncogene in breast cancer

Cancer Cell , 2014 Jun 16; 25(6):762-777

PubMed ID: 24937458 DOI: 10.1016/j.ccr.2014.04.024

Klein BJ, Piao L, Xi Y, Rincon-Arano H, Rothbart SB, Peng D, Wen H, Larson C, Zhang X, Zheng X, Cortazar MA, Peña PV, Mangan A, Bentley DL, Strahl BD, Groudine M, Li W, Shi X, Kutateladze TG

The histone-H3K4-specific demethylase KDM5B binds to its substrate and product through distinct PHD fingers.

Cell Rep , 2014 Jan 30; 6(2):325-35. Epub 2014 Jan 9

PubMed ID: 24412361 DOI: 10.1016/j.celrep.2013.12.021

Wouters J, Stas M, Gremeaux L, Govaere O, Van den broeck A, Maes H, Agostinis P, Roskams T, van den Oord JJ, Vankelecom H

The Human Melanoma Side Population Displays Molecular and Functional Characteristics of Enriched Chemoresistance and Tumorigenesis

PLoS One , 2013; 8(10):e76550. Epub 2013 Oct 3

PubMed ID: 24098529 DOI: 10.1371/journal.pone.0076550

Hayami S, Yoshimatsu M, Veerakumarasivam A, Unoki M, Iwai Y, Tsunoda T, Field HI, Kelly JD, Neal DE, Yamaue H, Ponder BA, Nakamura Y, Hamamoto R

Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway

Mol Cancer , 2010 Mar 13; 9:59. Epub 2010 Mar 13

PubMed ID: 20226085 DOI: 10.1186/1476-4598-9-59

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