Enhanced Validation

Anti-ITGB4 Antibody

integrin, beta 4
Recommended Applications
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Product Description
Polyclonal Antibody against Human ITGB4
Alternative Gene Names
CD104
Price
$445.00
Product Number
HPA036348
Unit Size
100 µl
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
integrin, beta 4
Target Gene
ITGB4
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
MKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYDSFLMYSDDVLRS
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000020758 (97%)

Rat ENSRNOG00000005580 (97%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Western Blot (WB)

Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.

Protein Name
integrin, beta 4
Gene Name
ITGB4
Alternative Gene Names
CD104
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000132470 on the Human Protein Atlas

Arun AS, Tepper CG, Lam KS

Identification of integrin drug targets for 17 solid tumor types

Oncotarget , 2018 Jul 10; 9(53):30146-30162. Epub 2018 Jul 10

PubMed ID: 30046394 DOI: 10.18632/oncotarget.25731

Wang J, Zhang B, Meng J, Xiao G, Li X, Li G, Qin S, Du N, Zhang J, Zhang J, Xu C, Tang SC, Liang R, Ren H, Sun X

Analysis of risk factors for post-operative complications and prognostic predictors of disease recurrence following definitive treatment of patients with esophageal cancer from two medical centers in Northwest China

Exp Ther Med , 2017 Jul 25; 14(3):2584-2594. Epub 2017 Jul 25

PubMed ID: 28962198 DOI: 10.3892/etm.2017.4835

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