Enhanced Validation

Anti-IDO1 Antibody

indoleamine 2,3-dioxygenase 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human IDO1
Alternative Gene Names
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
indoleamine 2,3-dioxygenase 1
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000031551 (58%)

Rat ENSRNOG00000031189 (57%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Recombinant expression validation in WB using target protein overexpression.

Protein Name
indoleamine 2,3-dioxygenase 1
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000131203 on the Human Protein Atlas

Sailer V, Sailer U, Bawden EG, Zarbl R, Wiek C, Vogt TJ, Dietrich J, Loick S, Grünwald I, Toma M, Golletz C, Gerstner A, Kristiansen G, Bootz F, Scheckenbach K, Landsberg J, Dietrich D

DNA methylation of indoleamine 2,3-dioxygenase 1 (IDO1) in head and neck squamous cell carcinomas correlates with IDO1 expression, HPV status, patients’ survival, immune cell infiltrates, mutational load, and interferon γ signature

EBioMedicine , 2019 Oct 15; 48:341-352. Epub 2019 Oct 15

PubMed ID: 31628024 DOI: 10.1016/j.ebiom.2019.09.038

Zhou S, Zhao L, Liang Z, Liu S, Li Y, Liu S, Yang H, Liu M, Xi M

Indoleamine 2,3-dioxygenase 1 and Programmed Cell Death-ligand 1 Co-expression Predicts Poor Pathologic Response and Recurrence in Esophageal Squamous Cell Carcinoma after Neoadjuvant Chemoradiotherapy

Cancers (Basel) , 2019 Feb 1; 11(2):169. Epub 2019 Feb 1

PubMed ID: 30717285 DOI: 10.3390/cancers11020169

Mehra S, Alvarez X, Didier PJ, Doyle LA, Blanchard JL, Lackner AA, Kaushal D

Granuloma Correlates of Protection Against Tuberculosis and Mechanisms of Immune Modulation by Mycobacterium tuberculosis

J Infect Dis , 2013 Apr; 207(7):1115-1127. Epub 2012 Dec 18

PubMed ID: 23255564 DOI: 10.1093/infdis/jis778

Did we miss your publication?

Have you published using HPA023072? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

Read more

Corresponding Antigens


Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch