Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000020048 (90%)
Rat ENSRNOG00000026963 (87%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Proteomic Analyses Reveal High Expression of Decorin and Endoplasmin (HSP90B1) Are Associated with Breast Cancer Metastasis and Decreased Survival
PLoS One , 2012; 7(2):e30992. Epub 2012 Feb 20
Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis.
Lab Invest , 2012 Sep; 92(9):1374-85. Epub 2012 Jul 2
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