Enhanced Validation

Polyclonal Anti-HPRT1 Antibody

hypoxanthine phosphoribosyltransferase 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human HPRT1
Alternative Gene Names
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
hypoxanthine phosphoribosyltransferase 1
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000048561 (98%)

Mouse ENSMUSG00000025630 (98%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
hypoxanthine phosphoribosyltransferase 1
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000165704 on the Human Protein Atlas

Grønning AG, Doktor TK, Larsen SJ, Petersen US, Holm LL, Bruun GH, Hansen MB, Hartung AM, Baumbach J, Andresen BS

DeepCLIP: predicting the effect of mutations on protein–RNA binding with deep learning

Nucleic Acids Res , 2020 Jun 19; 48(13):7099-7118. Epub 2020 Jun 19

PubMed ID: 32558887 DOI: 10.1093/nar/gkaa530

Bruun GH, Bang JM, Christensen LL, Brøner S, Petersen US, Guerra B, Grønning AG, Doktor TK, Andresen BS

Blocking of an intronic splicing silencer completely rescues IKBKAP exon 20 splicing in familial dysautonomia patient cells

Nucleic Acids Res , 2018 May 14; 46(15):7938-7952. Epub 2018 May 14

PubMed ID: 29762696 DOI: 10.1093/nar/gky395

Bruun GH, Doktor TK, Borch-Jensen J, Masuda A, Krainer AR, Ohno K, Andresen BS

Global identification of hnRNP A1 binding sites for SSO-based splicing modulation

BMC Biol , 2016 Jul 5; 14:54. Epub 2016 Jul 5

PubMed ID: 27380775 DOI: 10.1186/s12915-016-0279-9

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Corresponding Antigens


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