Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000025630 (98%)
Rat ENSRNOG00000048561 (98%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
DeepCLIP: predicting the effect of mutations on protein–RNA binding with deep learning
Nucleic Acids Res , 2020 Jun 19; 48(13):7099-7118. Epub 2020 Jun 19
Blocking of an intronic splicing silencer completely rescues IKBKAP exon 20 splicing in familial dysautonomia patient cells
Nucleic Acids Res , 2018 May 14; 46(15):7938-7952. Epub 2018 May 14
Global identification of hnRNP A1 binding sites for SSO-based splicing modulation
BMC Biol , 2016 Jul 5; 14:54. Epub 2016 Jul 5
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