Enhanced Validation

Anti-HNRNPR Antibody

heterogeneous nuclear ribonucleoprotein R
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human HNRNPR
Alternative Gene Names
hnRNP-R, HNRPR
Price
$445.00
Product Number
HPA026092
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
heterogeneous nuclear ribonucleoprotein R
Target Gene
HNRNPR
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
MANQVNGNAVQLKEEEEPMDTSSVTHTEHYKTLIEAGLPQKVAERLDEIFQT
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000066037 (100%)

Rat ENSRNOG00000011910 (98%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
heterogeneous nuclear ribonucleoprotein R
Gene Name
HNRNPR
Alternative Gene Names
hnRNP-R, HNRPR
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000125944 on the Human Protein Atlas

Duijkers FA, McDonald A, Janssens GE, Lezzerini M, Jongejan A, van Koningsbruggen S, Leeuwenburgh-Pronk WG, Wlodarski MW, Moutton S, Tran-Mau-Them F, Thauvin-Robinet C, Faivre L, Monaghan KG, Smol T, Boute-Benejean O, Ladda RL, Sell SL, Bruel AL, Houtkooper RH, MacInnes AW

HNRNPR Variants that Impair Homeobox Gene Expression Drive Developmental Disorders in Humans

Am J Hum Genet , 2019 May 9; 104(6):1040-1059. Epub 2019 May 9

PubMed ID: 31079900 DOI: 10.1016/j.ajhg.2019.03.024

Dombert B, Sivadasan R, Simon CM, Jablonka S, Sendtner M

Presynaptic Localization of Smn and hnRNP R in Axon Terminals of Embryonic and Postnatal Mouse Motoneurons

PLoS One , 2014; 9(10):e110846. Epub 2014 Oct 22

PubMed ID: 25338097 DOI: 10.1371/journal.pone.0110846

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Corresponding Antigens

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