Polyclonal Anti-HMCES Antibody
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000010474 (88%)
Mouse ENSMUSG00000030060 (87%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Loss of the abasic site sensor HMCES is synthetic lethal with the activity of the APOBEC3A cytosine deaminase in cancer cells
PLoS Biol , 2021 Mar 31; 19(3):e3001176. Epub 2021 Mar 31
HMCES Maintains Replication Fork Progression and Prevents Double-Strand Breaks in Response to APOBEC Deamination and Abasic Site Formation
Cell Rep , 2020 Jun 2; 31(9):107705
HMCES safeguards replication from oxidative stress and ensures error‐free repair
EMBO Rep , 2020 Apr 19; 21(6):e49123. Epub 2020 Apr 19
HMCES maintains genome integrity by shielding abasic sites in single strand DNA
Cell , 2018 Dec 13; 176(1-2):144-153.e13. Epub 2018 Dec 13
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.