Enhanced Validation

Polyclonal Anti-GSPT1 Antibody

G1 to S phase transition 1
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human GSPT1
Alternative Gene Names
eRF3a, ETF3A, GST1
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
G1 to S phase transition 1
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000046271 (74%)

Mouse ENSMUSG00000062203 (66%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
G1 to S phase transition 1
Gene Name
Alternative Gene Names
eRF3a, ETF3A, GST1
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000103342 on the Human Protein Atlas

Sialana FJ, Roumeliotis TI, Bouguenina H, Chan Wah Hak L, Wang H, Caldwell J, Collins I, Chopra R, Choudhary JS

SimPLIT: Simplified Sample Preparation for Large-Scale Isobaric Tagging Proteomics

J Proteome Res , 2022 Jul 18; 21(8):1842-1856. Epub 2022 Jul 18

PubMed ID: 35848491 DOI: 10.1021/acs.jproteome.2c00092

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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