Enhanced Validation

Polyclonal Anti-GSPT1 Antibody

G1 to S phase transition 1
References (2)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human GSPT1
Alternative Gene Names
eRF3a, ETF3A, GST1
Open Datasheet
Price
$505.00
Product Number
HPA052488
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemical staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.
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[1 pictures]
Western blot analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GSPT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PARP1.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
G1 to S phase transition 1
Target Gene
GSPT1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
GGAANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVS
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000046271 (74%)

Mouse ENSMUSG00000062203 (66%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for HPA052488 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
G1 to S phase transition 1
Gene Name
GSPT1
Alternative Gene Names
eRF3a, ETF3A, GST1
UniProt ID
P15170
Gene (Ensembl)
ENSG00000103342
Entrez Gene ID
Entrez gene: 2935
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000103342 on the Human Protein Atlas

Sialana FJ, Roumeliotis TI, Bouguenina H, Chan Wah Hak L, Wang H, Caldwell J, Collins I, Chopra R, Choudhary JS

SimPLIT: Simplified Sample Preparation for Large-Scale Isobaric Tagging Proteomics

J Proteome Res , 2022 Jul 18; 21(8):1842-1856. Epub 2022 Jul 18

PubMed ID: 35848491 DOI: 10.1021/acs.jproteome.2c00092

Did we miss your publication?

Have you published using HPA052488? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-GSPT1 (AMAb91849)
Anti-GSPT1 (AMAb91851)
Anti-GSPT1 (HPA074588)
Corresponding Antigens
PrEST Antigen GSPT1 (APrEST82187)

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