Enhanced Validation

Polyclonal Anti-GSDMD Antibody

gasdermin D
References (4)
Recommended Applications
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human GSDMD
Alternative Gene Names
DF5L, FLJ12150, GSDMDC1
Open Datasheet
Price
$505.00
Product Number
HPA044487
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[1 pictures]
Western blot analysis in human cell lines U2OS and HEK293 using Anti-GSDMD antibody. Corresponding GSDMD RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
gasdermin D
Target Gene
GSDMD
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
GDNVYVVTEVLQTQKEVEVTRTHKREGSGRFSLPGATCLQGEGQGHLSQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRSTS
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000007728 (63%)

Mouse ENSMUSG00000022575 (61%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
View all protocols
Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Protein Name
gasdermin D
Gene Name
GSDMD
Alternative Gene Names
DF5L, FLJ12150, GSDMDC1
UniProt ID
P57764
Gene (Ensembl)
ENSG00000104518
Entrez Gene ID
Entrez gene: 79792
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000104518 on the Human Protein Atlas

Theobald SJ, Gräb J, Fritsch M, Suárez I, Eisfeld HS, Winter S, Koch M, Hölscher C, Pasparakis M, Kashkar H, Rybniker J

Gasdermin D mediates host cell death but not interleukin-1β secretion in Mycobacterium tuberculosis-infected macrophages

Cell Death Discov , 2021 Oct 30; 7:327. Epub 2021 Oct 30

PubMed ID: 34718331 DOI: 10.1038/s41420-021-00716-5

Zhou B, Abbott DW

Gasdermin E permits interleukin-1 beta release in distinct sublytic and pyroptotic phases

Cell Rep , 2021 Apr 13; 35(2):108998

PubMed ID: 33852854 DOI: 10.1016/j.celrep.2021.108998

Rathkey JK, Xiao TS, Abbott DW

Human polymorphisms in GSDMD alter the inflammatory response

J Biol Chem , 2020 Jan 27; 295(10):3228-3238. Epub 2020 Jan 27

PubMed ID: 31988247 DOI: 10.1074/jbc.RA119.010604

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Corresponding Antigens
PrEST Antigen GSDMD (APrEST76404)

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