Enhanced Validation

Anti-GLUL Antibody

glutamate-ammonia ligase
Recommended Applications
Recombinant expression validation in WB using target protein overexpression.
Product Description
Polyclonal Antibody against Human GLUL
Alternative Gene Names
GLNS
Price
$445.00
Product Number
HPA007316
Unit Size
100 µl
Concentration
Lot dependent
Availability
6 in Stock
Ships
Ready to Ship
Target Protein
glutamate-ammonia ligase
Target Gene
GLUL
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
LQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGP
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000026473 (95%)

Rat ENSRNOG00000049560 (92%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
Western Blot (WB)

Recombinant expression validation in WB using target protein overexpression.

Protein Name
glutamate-ammonia ligase
Gene Name
GLUL
Alternative Gene Names
GLNS
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000135821 on the Human Protein Atlas

Kitajima S, Lee KL, Hikasa H, Sun W, Huang RY, Yang H, Matsunaga S, Yamaguchi T, Araki M, Kato H, Poellinger L

Hypoxia-inducible factor-1α promotes cell survival during ammonia stress response in ovarian cancer stem-like cells

Oncotarget , 2017 Dec 7; 8(70):114481-114494. Epub 2017 Dec 7

PubMed ID: 29383096 DOI: 10.18632/oncotarget.23010

Perisic L, Hedin E, Razuvaev A, Lengquist M, Osterholm C, Folkersen L, Gillgren P, Paulsson-Berne G, Ponten F, Odeberg J, Hedin U

Profiling of atherosclerotic lesions by gene and tissue microarrays reveals PCSK6 as a novel protease in unstable carotid atherosclerosis.

Arterioscler Thromb Vasc Biol , 2013 Oct; 33(10):2432-43. Epub 2013 Aug 1

PubMed ID: 23908247 DOI: 10.1161/ATVBAHA.113.301743

Ko YH, Lin Z, Flomenberg N, Pestell RG, Howell A, Sotgia F, Lisanti MP, Martinez-Outschoorn UE

Glutamine fuels a vicious cycle of autophagy in the tumor stroma and oxidative mitochondrial metabolism in epithelial cancer cells: Implications for preventing chemotherapy resistance

Cancer Biol Ther , 2011 Dec 15; 12(12):1085-1097. Epub 2011 Dec 15

PubMed ID: 22236876 DOI: 10.4161/cbt.12.12.18671

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