Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Hepatic miR-215 target Rictor and modulation of hepatic insulin signalling in rats
Mol Med Rep , 2019 Mar 14; 19(5):3723-3731. Epub 2019 Mar 14
Effect of eNOS on Ischemic Postconditioning-Induced Autophagy against Ischemia/Reperfusion Injury in Mice
Biomed Res Int , 2019 Feb 10; 2019:5201014. Epub 2019 Feb 10
Moderate activation of IKK2-NF-kB in unstressed adult mouse liver induces cytoprotective genes and lipogenesis without apparent signs of inflammation or fibrosis
BMC Gastroenterol , 2015 Jul 30; 15:94. Epub 2015 Jul 30
C11orf83, a Mitochondrial Cardiolipin-Binding Protein Involved in bc1 Complex Assembly and Supercomplex Stabilization
Mol Cell Biol , 2015 Apr; 35(7):1139-1156. Epub 2015 Mar 10
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